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Decay models
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- Multi-exponential model for fluorescence and anisotropy decays (terminal model)
- Fractal Forster energy transfer model for fluorescence and anisotropy decays (terminal model)
- Arbitrary (additive) combination of the terminal models
- Associative and non-associative models for anisotropy
- Any combination of fluorescence and anisotropy parts of different terminal models
Modular object-oriented architecture allows for easy extension of the model library
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Instrumental models
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- Instrumental response function via scattering solution
- Instrumental response function via single exponential reference compound
- Background and scattered light
- Time shift
- Anisotropy G-factor
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Methods
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- Global fit: several fluorescence decays are combined and simultaneously fitted
- Anisotropy analysis via simultaneous fit of parallel and perpendicular components of fluorescence
- Automatically generated initial guesses for parameters
- Parameter fixing, constraints and linkage
- Confidence intervals by exhaustive search and standard errors
- Quality of fit is judged by chi-square criterion, visual inspection of residuals and auto-correlation function of residuals
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Interface
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- Multi-document interface
- Advanced parameters management (sorting, quick linkage and easy navigation)
- Templates for quick saving and restoring analysis configurations
- Saving and loading experimental data and analysis results from databases
- 2D and 3D graphical data representation
- Import of external data and export of the results of analysis
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Databases
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- Storing and managing experimental data, templates and analysis results
- Searching, sorting and filtering data
- Support of data integrity and validity
- Avoiding of data redundancy
- Report generation
- 2D graphical data previewing
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