FFS Data Processor. List of updates:


FFS Data Processor 2.7  (03.12.19)

Measurements database:

1. Import and processing of raw data (*spc files) of Becker & Hickl (B&H) cards SPC-140, SPC-144, SPC-150, SPC-154 is added;

2. Import and processing of raw data (*spc files) of Becker & Hickl (B&H) cards SPC-142, SPC-152 is added;

3. Add Files Group Wizard is extended by the possibility to copy all settings and parameters from the current record. It allows to import data much more quickly using this wizard;


FFS Data Processor 2.7  (22.08.19)

Main application:

1. Brightness correction in all FCS models (Triplet State, Conformational, ...) is extended to support also brightness ratio. It opens the possibility to perform the global analysis of ACFs and PCDs in a case if the brightness ratio q2/q1, q3/q1, ... is selected in the PCH model. The brightness ratio can be either fixed to know value or constrained to some reasonable boundaries that enhance the performance of the iterative fitting;

2. Improvement of automated creation of a global ACF + PCD analysis Three options, the type of the brightness correction, the dead time correction and fixation of the infinity term to unity were added. It allows to configure the analysis in details directly while its creation;


Measurements database:

1. Enhancement of the Raw Data Builder capabilities in a way of creation and handling of a big number of measured raw data. Automated creation of a set of curves (characteristics) ACFs, PCDs, FFCs with predefined parameters is programmed. It means one can initially define what characteristics one wants to calculate, define all needed parameters such as number of channels and time step, and finally calculate a number of sets of characteristics (e.g. at different time ranges in the raw data) one after another by clicking just one button. It allows to calculate coincident sets of ACFs + PCDs for their subsequent global analysis very quickly. One can also update the set of already calculated characteristics very quickly after e.g. changing the time ranges in the raw data. It is especially important in the analysis of data measured in living cells where just parts of measured traces are stationary and suitable for the analysis.

2. The naming newly calculated characteristics policy is changing. More options are added to the name that allows to sort them quickly.

3. Synchronization of calculated characteristics with the raw data from which they were calculated (while navigation among them) is added.


FFS Data Processor 2.6  (28.10.15)

Main application:

1. Automated creation of a global ACF + PCD analysis (FCS+PCH global analysis). Creation of necessary parameter groups and linking is done automatically;

2. Plotting of fit parameter histograms;

3. Normalization of ACFs is now performed by the maximum of correspondent theoretical curves (if they are available, i.e. after analysis);

4. The possibility to select the target chi2 criteria, standard global or weighted global (accordingly to Skakun et.al. 2011) is added;

5. The location (in Configuration dialog) of previously analysed data is memorized in registry;

6. The location of previously opened analysis (in Open/Save Analysis dialog) is memorized in registry;

7. Stored data are now listed in descending order (last analysis is first in the list) in Open/Save Analysis dialog.


Measurements database:

Direct (non-iterative) fast estimation of model parameters of measured (or calculated from raw data files) data. Fast estimation of model parameters is available for auto(cross)correlations, photon counting distributions and fluorescence cumulants. Triplet-state model is the default model for auto(cross)correlations, PCH with 1st order out-of-focus correction is used for photon counting distributions and FCA with 1st order out-of-focus correction is used for fluorescent cumulants (actually the same algorithm is used for parameter estimation for two last models).


FFS Data Processor 2.5  (16.02.15)

Measurements database:

1. Improved algorithm of bursts selection (in the intensity trace);

2. Bursts analysis. A possibility to select bursts in addition to reject bursts is added, i.e. to calculate ACF, PCD, etc. curves from the selected bursts (one curve per each burst);

3. Calculation of averaged ACF in a case of bursts analysis;

4. Possibility to interrupt the calculation of ACF, PCD, etc. curves in a case of processing of multiple raw data files, bursts analysis and if Multiple characteristic calculation regime is activated (calculation with increased bin time and sliding analysis);

5. Selection of time unit while import of already calculated curves (ACF, etc.) stored primarily in text files. Note: Data processor is independent from time dimension, it estimates parameters accordingly to the imported data. It is mostly for convenience purposes because lots of things (min/max settings, etc. are initially set assuming time in ms);

6. Acceleration of raw data processing in a case of calculation of ACF, PCD, etc. from parts of data (from bursts, in sliding regime);

7. Memorization of user settings in Raw data builder and Burst selection/rejection forms and in the Import data wizard;

8. Auto edit tables mode id added. It allows to edit all database tables without necessity to click Edit button in the database navigator each time before editing;

9. Enabling of resizing of Open and Save data dialogues;

10. Important. A data dependent problem in a calculation of crosscorrelation from two channel Confocor 2 raw data is fixed. This bug was introduced in the last versions. If a calculation of crosscorrelation from Confocor 2 raw data was interrupted with the error "Flow is incorrect" (this error is shown if next photon arrives before the current one), most probably you met this problem. In some cases depending on data some photons might be accounted in a wrong channel (respectively with assignment of arrival time which differ from the actual one in 1 - 3 clock-time events). Please ask for a free update of the Measurements database if you are working with two channel Confocor 2 data.


Analysis database:

1. Joining of several analyses stored in the database. Several analyses performed on the same sample (from repetitions, kinetic measurement, etc.) can now be joined in one analysis. After joining of global parameters (instrumental parameters and others expected to have same value across all analysis) one gets a 'super' global analysis;

2. Calculation of histograms of fit parameters is now possible;

3. Linking to other databases like it was done in the Measurements database is now possible also in the Analysis database.


FFS Data Processor 2.4  (25.10.13)

1. Direct (i.e. without merging) import, processing and analysis of data from two SPC 130 cards (SPC 132);

2. Calculation of red-to-blue (in addition to blue-to-red) crosscorrelation functions from the raw data. Calculation of averaged (blue-to-red + red-to-blue)/2 crosscorrelation function;

3. Burst rejection is updated by introducing user-defined gaps placed before and after of each selected busts. Vertical thresholds values can now be set by user. All settings are memorized in regestry.


FFS Data Processor 2.3  (20.05.13)

1. Import of new PiqoQuant Symphotime 64 (*.ptu) data is implemented.


FFS Data Processor 2.3  (02.02.12)

1. Import of 5 column ASCII data (X, Y channel red, Y channel red weight, Y channel blue, Y channel blue weight) is implemented;

2. Import of 7 column ASCII cross-correlation data (time-lag, ACF red, ACF red weight, ACF blue, ACF blue weight, CCF, CCF weight) is implemented;

3. Analysis of ICS (time correlation) data is now possible. Import of time correlation data exported from ImFCS software into three ASCII files: one column time-lag file, N-colunm ACF file, 2*N-column SD+AVG files is implemented;

4. Import/Export analyses stored in the Analysis database is now possible.


FFS Data Processor 2.3  (06.01.11)

1. Import of PicoQuant PicoHarp 300 data without the header (when the equipment is controlled LabView interface) is implemented;

2. Calculation of the Normalization value (required for PCD analysis) from data imported from the text files is added. See also the local menu item of the Files grid of the Measurement database.


FFS Data Processor 2.3  (01.11.10)

1. PCH model was extended:


2. Analysis and manipulation of a large number of fitted curves is improved:


3. Other changes:


4. Measurements database was updated:


FFS Data Processor 2.2  (01.06.09)

PCH model was updated. A ratio of both number of molecules and brightness could now be fitted for a two and more components model, i.e. a list of four parameter combinations is supported: N1, q1, N2, q2,…; N1, q1, N2, q2/q1,…; N1, q1, N2/ N1, q2,…; N1, q1, N2/ N1, q2/q1,…;


FFS Data Processor 2.1  (15.09.08)

Import of simple raw data formats is updated. Not it is possible to import raw data (sequence of photon arrival times) stored in TEXT files. One channel data and data with router are supported.

FRD.dll is updated.


FFS Data Processor 2.1  (01.05.08)

Import and processing of raw data of PicoQuant PicoHarp 300 and PicoQuant Time Harp 200 dataformats is supported.

Import of SIN files is implemented.

The problem with global analysis of a large number of datasets having large number of estimated parameters (usually leads to the error EOverflow) is fixed.

The problem with freezing of global analysis at the end is fixed. These problems were related to calculation of inverse Marquardt matrix of large dimension. Now it is calculated only when necessary (for the calculation of standard errors of estimated parameters and calculation of weight factors while secondary characteristic building).


FFS Data Processor 2.0  (16.04.07)

New methods:

PCH with correction for the out-of-focus emission

Following observation profiles are supported:

Following corrections are supported:

IG (method of moments) were developed for all types of observation profiles. This allows to fit data in just a few iterations.

TIFCA (according to Wu et al., 2005).

Following observation profiles are supported:

Following corrections are supported:

IG for all cases were developed (method of moments)


Main updates:

1. In addition to Global Analysis the Sequential fit of a number of datasets was implemented (see property Analysis Type of the Experiment. It can be set to either Global or Sequential).

2. Calculation and viewing of the local Target Criterion Value (TFC) (ChiSquare) for all data sets was implemented. It now allows to explore quality of fit of an individual dataset in the global analysis.

3. Automatic calculation of a number of any characteristics with different bin time (Time Step) from a number of raw data files was implemented.

4. Creation of a Fit Parameter Dependence (FPD) curve from a number of Experiments (opened experiment windows). The FPD was a dependence of certain fit parameter (for example brightness) on the external parameter (for example bin time). Weighting factors are assumed to be asymptotic errors of fit parameters derived from diagonal elements of variance-covariance matrix. The FPD can be created by using the interface elements of "Experiment configuration window" located on the page "Fit parameter dependence". Created FDPs can be added to the experiment and analyzed by the User-created model.

5. Import of ASCII file that contain of any column organized data was implemented. Possibility to choose separator, delimiter, start/end row, data type for each column is supported.

6. Calculation of crosscorrelation function with leaking correction was implemented.

7. The brightness parameters are available for the fit when the brightness correction is set on for FCS models.

8. The possibility to generate Initial Guesses, calculate TFC and analyze individual data set within the experiment was added. These options are available for the case of sequential analysis and can be accessed trough local menu of "Experiment configuration window".

9. Import of Confocor 3 raw data.

10. Rejection of unwanted bursts of intensity was implemented.

11. Simulator of Poisson and double stochastic Poisson (with exponentially decaying autocorrelation function) random point processes was implemented. It allows to simulate raw data with user-defined properties.

Fields "Fit Criterion" and "Analyzed" were added in the analysis database. It requires restructuring the analysis database. 


FCS Data processor was extended and renamed to FFS Data processor  (01.01.07)


FCS Data processor 1.4.2  (25.02.06)

1. Multicolumn data import was implemented;

2. Bugs with confidential intervals analysis and calculation of Chi2 were fixed;

3. 3D chart visualization bugs were fixed;

4. Automatic creation of statistical characteristics with varying TimeStep was implemented.


FCS Data processor 1.4.1 (01.04.05)

Main updates:

  1. Models library for fitting auto(cross)correlation functions has been extended:

·        correction for brightness difference is added;

·        correction for background is added;

·        2D free diffusion type is added to the models;

·        2D and 3D anomalous diffusion types are added to the models;

·        2D and 3D confined diffusion types are added;

·        flow model is added;

·        Ginf (the level of autocorrelation function at infinity) parameter is added to all models;

·        any combinations of processes (flow, triplet intercrossing, conformational changes and protonation) and motion terms (2D/3D free, anomalous and confined diffusion) are now possible;

  1. Fluorescence Cumulants Analysis (FCA) is implemented. Observation profile shape factors can be calculated for 2D/3D Gaussian and squared Gaussian Lorenzian functions;
  2. Coincidence analysis is implemented. Coincidence values histogram can be fitted by Gaussian model and separation quality parameter can be calculated;
  3. Coincidence bursts selection and counting is implemented.
  4. Support of following raw data formats: ISS ALBA, B&H SPC 4x1/4x2, 6x0 256/4096ch, 134, 830 is added


Updates of Data Processor:

  1. Keeping user-defined size and position of most useful windows;
  2. Selection of required data set by double clicking left mouse button on the corresponding curve displayed on 2D chart;
  3. Saving and restoring user-defined settings of 2D and 3D charts;
  4. Improved 3D charts based on Microsoft DirectX technology;
  5. Improved configuration dialog for 3D charts;
  6. A window for estimating separation of coincidence values histograms;
  7. Improved script editor for custom model;
  8. The following functions for writing Custom model scripts were added: error function, complementary error function, gamma function, natural logarithm of the gamma function;
  9. The Wizard for step-by-step creation of custom models.


Updates of Measurements Database:

  1. Bursts selection and counting
  2. Import a series of raw data files (usually repetitions of the measurement) at once
  3. Processing a series of raw data files at once, i.e. calculation of the same characteristic from each raw data file automatically
  4. Calculation of Intensity, Fluorescence Factorial Cumulants, Coincidence Values Histogram from raw data
  5. Calculation of logarithmic auto(cross)correlation function without averaging, i.e. with the same bin time for all quasi-logarithmically spaced time lags
  6. Saving filter expressions after closing the application
  7. Previewing of weight factors, i.e. standard deviations and relative standard deviations of each point of the characteristic
  8. Extending of chart configuration features: saving user defined settings, automatic (depend on viewed characteristic) and manual setting of linear/logarithmic scale of X, Y axis and their titles
  9. Calculation of a number of photons inside a specified time region


Updates of Analysis Database:

  1. Previewing of parameters values vs. analysis ID
  2. Saving filter expressions after closing the application
  3. Extending of chart configuration features. Saving user defined settings