The present software is a simplified, but general purpose, module that uses the Maximum Entropy Method to analyse the fluorescence kinetic data. It is written in Java in order to run on different platforms.
The software was developed by Eugene Novikov in 2002, when he was working together with Daniel Lavalette in the Molecular Biophysics Department of the Institute Curie in Orsay, France. At the moment, with permission of the developer, the software is supported and updated by SSTC team. For any questions regarding the software please contact us.
The software package has been developed as a cross-platform envelope for the MemSys5 routine (Maximum Entropy Data Consultants Ltd) facilitating its use in biophysical applications. The MemSys5 routine had been originally delivered in FORTRAN. Therefore, to avoid any platform-specific features, MemSys5 had also been rewritten in Java. Besides import/export, data visualization and basic MEM-processing functionality, the software includes a simulation module to mimic real kinetics data. The MEM User Manual and MEM Video Manual (AVI, 25.6 MB) explains in detail the structure and the use of the software.
The software is delivered as a stand-alone compressed Java jar file, which nowadays can be run on virtually all machines and operating systems. First, one has to install Java Runtime Environment (JRE) or Java Development Kit (JDK), freely available on https://java.com/en/download/, before running the software.
The analysed data is not allowed to contain a negative amplitude. Therefore, a rising part such as the buildup of acceptor fluorescence upon donor excitation in FRET systems cannot be treated with this version. In addition, the software does not provide for deconvolution from the impulse response function. Here, the impulse response function is assumed to be a delta function.
The input file with the kinetic data should have three tab-separated columns with a one-line header as shown below for three data points:
The software is offered freely to the research community in order to promote and popularize the
powerful and rigorous MEM approach. In spite of porting MemSys5 routine to Java this is a co-called derivative work and the license to MemSys5 is still required.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND. IN NO EVENT SHALL THE DEVELOPERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, ARISING FROM THE USE OF THE SOFTWARE.
To download the software use the provided link.
For any work reporting results obtained by firstMEM software you should use one of the following citations:
History of updates: