Data Management
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Import of B&H SPC data (up to four channels)

Import of PicoQuant TimeHarp 200, PicoHarp 300 and Symphotime64bit (up to four channels)

Calculation of absolute photon arrival times when possible
Macro & micro
time processing
Automatic and manual
Burst selection in the intensity and time lag plot
Sliding by either
selected bursts or constant time or constant amount of photons
Calculation of
fluorescence decays and anisotropy
Calculation of autocorrelation functions (with and without the normalization)
and inter arrival time distribution in linear and quasilogarithmic time scales
Binning of fluorescence decays
Calculation and
plotting Energy Transfer Efficiency and Fractional Intensity for two
color data
Calculation and
plotting Steady State Polarization and Anisotropy for polarized data
Selecting
userdefined time regions, start and end analysis channels with
cursors
Displaying
fluorescence and triplet lifetimes histogram
Export data to ASCII
file

Export autocorrelation functions to
ConfoCor formatted
file
Analysis Methods
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Sliding
analysis (Maximum Likelihood with multinomial statistics and
LevenbergMarquardt optimization algorithm)
Analysis
of selected dataset
Automatic
shift detection
Fit
with and without convolution
Correction
for the background
Automatically
generated initial guesses
Parameter
fixing and constraints
Confidence
intervals by standard errors
Quality
of fit: MLE criterion value and visual inspection of residuals and
autocorrelation of residuals
FCS
analysis

IATD coincidence
analysis
Analysis
Models
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Fluorescence
decay analysis
where:
– d
is time shift;
– b
is level of dark noise;
– g
is background multiplication factor;
– с
timeuncorrelated background;
– irf(t)
is measured instrument response function;
– bg(t)
is measured background (optional);
Anisotropy
analysis
where:
–
r_{inf} is anisotropy when t → ∞;
– r_{j}
are preexponential factors;
– j_{j}
are rotational correlation times;
– M
is number of exponents.
Analysis
of autocorrelation function for immobilized molecules
where:
–
c_{j} are preexponential factors;
– t_{j}
are tripletstate lifetimes;
– K
is number of exponents.
Analysis
of autocorrelation function for molecules in solution (FCS)
where:
–
F and t are
tripletstate fraction and lifetime;
– N
is an average number of molecules in observation volume;
– F_{j}
are fractions of corresponding diffusion components;
– T_{Dj}
are diffusion times of corresponding diffusion components;
Model library is easily extendable